BIOFILM PRODUCTION AND ANTIBACTERIAL ACTIVITY OF THE ESSENTIAL OIL OF MENTHA PIPERITA AGAINST STAPHYLOCOCCUS AUREUS ISOLATED FROM PATIENTS WITH CHRONIC RHINOSINUSITIS
DOI:
https://doi.org/10.47820/jht.v2i1.32Keywords:
Sinusitis, Nasal Polyp, Biofilms, Mentha piperita, Staphylococcus aureusAbstract
Background: Chronic Rhinosinusitis (CRS) is an inflammatory disease of the mucosa of the nasal cavity and paranasal sinuses. The Staphylococcus aureus (S. aureus) are frequently found in patients with CRS and biofilms from this species are often associated with recalcitrant or recurrent disease. Objective: To verify the formation of biofilm in S. aureus carried by patients with Chronic Rhinosinusitis with Nasosinusal Polyposis (CRSwNP) and to perform in vitro tests to evaluate the antimicrobial capacity of the essential oil of Mentha piperita (M. piperita). Methods: Foram analisadas 14 amostras de S. aureus isolados oriundos de pacientes com CRSwNP, atendidos no ambulatório de otorrinolaringologia do Regional Hospital de Presidente Prudente- SP. Samples were seeded on blood agar and subjected to Gram, catalase and coagulase stains. To verify the production of biofilm, we used the methods of adherence in tubes of borosilicate and Congo red agar, as well as, the antimicrobial activity of the essential oil of M. piperita was evaluated through the technique of Minimum Inhibitory Concentration (MIC). Results: The results indicated that the analyzed S. aureus has the ability to form a biofilm and that the essential oil of M. piperita is effective in inhibiting the growth of this bacterium according to the concentrations used. Conclusion: In view of the results obtained, we concluded that the S. aureus isolated from patients with CRSwNP have the ability to form a biofilm and that the essential oil of M. piperita has antimicrobial potential on the tested strains.
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