BIOFILM PRODUCTION AND ANTIBACTERIAL ACTIVITY OF THE ESSENTIAL OIL OF MENTHA PIPERITA AGAINST STAPHYLOCOCCUS AUREUS ISOLATED FROM PATIENTS WITH CHRONIC RHINOSINUSITIS
DOI:
https://doi.org/10.47820/jht.v2i1.32Keywords:
Sinusitis, Nasal Polyp, Biofilms, Mentha piperita, Staphylococcus aureusAbstract
Background: Chronic Rhinosinusitis (CRS) is an inflammatory disease of the mucosa of the nasal cavity and paranasal sinuses. The Staphylococcus aureus (S. aureus) are frequently found in patients with CRS and biofilms from this species are often associated with recalcitrant or recurrent disease. Objective: To verify the formation of biofilm in S. aureus carried by patients with Chronic Rhinosinusitis with Nasosinusal Polyposis (CRSwNP) and to perform in vitro tests to evaluate the antimicrobial capacity of the essential oil of Mentha piperita (M. piperita). Methods: Foram analisadas 14 amostras de S. aureus isolados oriundos de pacientes com CRSwNP, atendidos no ambulatório de otorrinolaringologia do Regional Hospital de Presidente Prudente- SP. Samples were seeded on blood agar and subjected to Gram, catalase and coagulase stains. To verify the production of biofilm, we used the methods of adherence in tubes of borosilicate and Congo red agar, as well as, the antimicrobial activity of the essential oil of M. piperita was evaluated through the technique of Minimum Inhibitory Concentration (MIC). Results: The results indicated that the analyzed S. aureus has the ability to form a biofilm and that the essential oil of M. piperita is effective in inhibiting the growth of this bacterium according to the concentrations used. Conclusion: In view of the results obtained, we concluded that the S. aureus isolated from patients with CRSwNP have the ability to form a biofilm and that the essential oil of M. piperita has antimicrobial potential on the tested strains.
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Kim D, Assiri AM, Kim JH. Recent Trends in Bacteriology of Adult Patients with Chronic Rhinosinusitis. Journal of Clinical Medicine 2019, Vol 8, Page 1889 2019;8:1889. https://doi.org/10.3390/JCM8111889. DOI: https://doi.org/10.3390/jcm8111889
Poddighe D, Vangelista L. Staphylococcus aureus Infection and Persistence in Chronic Rhinosinusitis: Focus on Leukocidin ED. Toxins (Basel) 2020;12:678. https://doi.org/10.3390/TOXINS12110678. DOI: https://doi.org/10.3390/toxins12110678
Santos AL sos, Santos DO, Freitas CC de, Ferreira BLA, Afonso IF, Rodrigues CR, et al. Staphylococcus aureus: visitando uma cepa de importância hospitalar. J Bras Patol Med Lab 2007;43:413–23. https://doi.org/10.1590/S1676-24442007000600005. DOI: https://doi.org/10.1590/S1676-24442007000600005
Christensen GD, Simpson WA, Younger JJ, Baddour LM, Barrett FF, Melton DM, et al. Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol 1985;22:996. https://doi.org/10.1128/JCM.22.6.996-1006.1985. DOI: https://doi.org/10.1128/jcm.22.6.996-1006.1985
CLSI C and LSI. M100-performance standards for antimicrobial susceptibility testing. vol. 28. CLINICAL AND LABORATORY; 2018.
Pombo JCP, Ribeiro ER, Pinto R de L, Silva BJM da. Efeito antimicrobiano e sinergístico de óleos essenciais sobre bactérias contaminantes de alimentos. Segurança Alimentar e Nutricional 2018;25:108–17. https://doi.org/10.20396/SAN.V25I2.8651785. DOI: https://doi.org/10.20396/san.v25i2.8651785
Capistrano ECJ. Avaliação da associação entre biofilmes bacterianos, bactérias intracelulares e superantígenos estafilocócicos em pacientes com rinossinusite crônica. Universidade de São Paulo, 2018. https://doi.org/10.11606/T.17.2018.TDE-10042018-141217. DOI: https://doi.org/10.11606/T.17.2018.tde-10042018-141217
Bezerra TFP. O papel do biofilme na rinossinusite crônica com polipose nasossinusal. Universidade de São Paulo, 2012. https://doi.org/10.11606/T.5.2012.TDE-01082012-135039. DOI: https://doi.org/10.11606/T.5.2012.tde-01082012-135039
Alves LA, Freires I de A, Castro R de D. Efeito antibacteriano de óleos essenciais sobre bactérias formadoras do biofilme dentário. Revista Brasileira de Ciências Da Saúde 2011;14:57–62. https://doi.org/10.4034/RBCS.2010.14.02.07. DOI: https://doi.org/10.4034/RBCS.2010.14.02.07
Nonato I da S. Efeito antibacteriano do óleo essencial de Mentha Piperita L. sobre as bactérias Sthaphylococcus aures e Escherichia coli. Faculdade Maria Milza, 2018.
Ferreira H. Avaliação da atividade antibacteriana de extratos de plantas medicinais: Significância sanitária em região de tríplice fronteira. Universidade de São Paulo, 2018.
Valeriano C, Piccoli RH, Cardoso MG, Alves E. Atividade antimicrobiana de óleos essenciais em bactérias patogênicas de origem alimentar. Revista Brasileira de Plantas Medicinais 2012;14:57–67. https://doi.org/10.1590/S1516-05722012000100009. DOI: https://doi.org/10.1590/S1516-05722012000100009
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